C are the absorbances of the sample and standard solutions respectively.
For assay of substance/s in multi component samples by spectrophotometer; the following methods are being used routinely, which includes , • Simultaneous equation method • Derivative spectrophotometric method • Absorbance ratio method (Q-Absorbance method) • Difference spectrophotometry • Solvent extraction method Paracetamol or acetaminophen is a widely used over-the-counter analgesic (pain reliever) and antipyretic (fever reducer).
"; var windowwidth = window.inner Width; var windowheight = window.inner Height; var print Win = window.open('','','left=0,top=0,width=' windowwidth ',height=' windowheight ',toolbar=0,scrollbars=0,status =0'); print Win.document.write(html); print Win.document.close(); print Win.focus(); print Win.print(); print Win.close(); } We protect the world's critical resources from harmful contamination.We aim to replace less sustainable chemical, thermal and filtration based technologies with ‘Application Optimised Ultraviolet (UV) light technology’, driven by unrivalled customer care.The assay of single component sample, which contains other absorbing substances, is then calculated from the measured absorbance by using one of three principal procedures.They are, use of standard absorptivity value, calibration graph and single or double point standardization.Mathematically, Beer- Lambert law is expressed as A=a b c Where, A=absorbance or optical density a=absorptivity or extinction coefficient b=path length of radiation through sample (cm) c=concentration of solute in solution.
Both b and a are constant so a is directly proportional to the concentration c When c is in gm/100 ml, then the constant is called A (1%, 1 cm) Quantification of medicinal substance using spectrophotometer may carried out by preparing solution in transparent solvent and measuring it’s absorbance at suitable wavelength.
Siladitya Behera Department of Quality Assurance and Pharma Regulatory Affairs Gupta College Of Technological Sciences Ashram More, G. Road Asanol-713301, Burdwan District West Bengal, India E-mail: [email protected] Received date: October 01, 2012; Accepted date: October 27, 2012; Published date: October 31, 2012 Citation: Behera S, Ghanty S, Ahmad F, Santra S, Banerjee S (2012) UV-Visible Spectrophotometric Method Development and Validation of Assay of Paracetamol Tablet Formulation. doi: 10.4172/2155-9872.1000151 Copyright: © 2012 Behera S, et al.
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Which does not shows any interference in spectrophotometric estimations.
All the parameters of the analysis were chosen according to ICH [Q2(R1)] guideline and validated statistically using RSD and %RSD along with neat chromate grams.
The single point standardization procedure involves the measurement of the absorbance of a sample solution and of a standard solution of the reference substance.